Protein Engineering
Protein Engineering Concepts in Protein Engineering Protein Structure and Function : Proteins are composed of amino acids and fold into specific three-dimensional structures, determining their function. Understanding the relationship between structure and function is crucial for effective engineering. Methods of Protein Engineering : Site-Directed Mutagenesis : Targeted changes to specific amino acids in a protein to study effects on function. Random Mutagenesis : Introducing random mutations to create a library of variants for screening. Directed Evolution : Iterative cycles of mutation and selection to evolve proteins with desirable traits. Computational Protein Design : Utilizing algorithms and simulations to predict how changes in amino acid sequences will affect protein structure and function. Tools like Rosetta and AlphaFold are increasingly used to model protein structures accurately. Applications of Protein Engineering Therapeutics : Designing proteins for targeted drug d...