Recombinant DNA (rDNA) technology
Recombinant DNA (rDNA) technology Recombinant DNA (rDNA) technology involves combining DNA from different sources to create new genetic combinations. This powerful tool has revolutionized biotechnology, medicine, and agriculture. Here’s an overview: Key Concepts of Recombinant DNA Technology Basic Principles : DNA Isolation : Extracting DNA from organisms. Cutting DNA : Using restriction enzymes to cut DNA at specific sequences, creating "sticky ends" for easy joining. Ligation : Joining the DNA fragments together using DNA ligase, forming a recombinant molecule. Vectors : Vectors are DNA molecules used to carry foreign genetic material into a host cell. Common vectors include plasmids, bacteriophages, and yeast artificial chromosomes (YACs). Transformation : The process of introducing the recombinant DNA into host cells (e.g., bacteria, yeast, or plant cells) so they can replicate and express the new genetic material. Selection and Screening : Selecting transformed cells t...