Biomolecules Biomolecules include both micromolecules, e.g. amino acids, nitrogenous bases, fatty acids, sugar, etc. and macromolecules, such as carbohydrates, proteins, lipids and nucleic acids. Living organisms are made up of organic as well as inorganic substances. For analysing the organic constituents of living tissue, we mix it with CCl 3 COOH (Trichloroacetic acid) and make a slurry by grinding. We get two fractions: Acid soluble fraction- Organic micromolecules (biomolecules) (mol wt. 18-800 Da) and inorganic compounds, e.g. phosphate, sulphate, etc. It mostly accounts for the cytoplasm Acid insoluble fraction- Polymeric macromolecules (mol wt. >10 thousand Da) and also lipid (a component of the cell membrane and form water-insoluble vesicles on fragmentation) The inorganic constituents can be estimated by analysing the ash formed after burning a tissue completely. All the macromolecules are homo or heteropolymers of various simple compounds. Protein- Polymer of amino ...